Biological effects of essential oils and extracts
Z. Kalvandi; J. Nazemi rafie; A. Sadeghi; A. Salimi; R. Kalvandi; M. Negahban
Abstract
Nowadays, the invention and application of agricultural pest control non-chemical methods is one of the important and practical ones in pest management programs in most societies of the world. Meanwhile, a large number of studies have been focused on bio-based pesticides. In this regard, new formulations ...
Read More
Nowadays, the invention and application of agricultural pest control non-chemical methods is one of the important and practical ones in pest management programs in most societies of the world. Meanwhile, a large number of studies have been focused on bio-based pesticides. In this regard, new formulations of plant insecticides have been developed as a suitable alternative to artificial pesticides. In this research, respiratory and contact toxicity of pure and formulated Eucalyptus globulus Labill. essential oil and respiratory toxicity durability of its essential oil nanocapsules on adult insects of Tribolium confusum Herbest. (Col., Tenebrionidae) were investigated under laboratory conditions. According to the results, for respiratory toxicity, the pure and formulated essential oil LC50 at 24 h was obtained 46.39 and 1472 μl.l-1 of air, respectively. The formulated essential oil LT50 in three concentrations of LC25, LC50, and LC90 was determined 12.3, 10.39, and 0.36 days, respectively. The results also showed that the contact toxicity of formulated essential oil (LC50= 19030 μl.l-1) on this insect was much higher than the contact toxicity of pure one (LC50= 3770 μl.l-1). Therefore, due to the good stability of the formulated essential oil in respiratory toxicity and the excellent effect in contact toxicity, after additional tests, eucalyptus essential oil nanocapsules could be used in the management of this important pest.
R. Kalvandi; M. Mirza; M. Atri; M. Hesamzadeh Hejazi; Z. Jamzad; K. Safikhani
Abstract
Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination ...
Read More
Thymus eriocalyx (Ronniger) Jalas belongs to the family Lamiaceae. Among different species of Thymus, distributed in the world, 18 species are growing in Iran, of which four species are endemic. To study the variation of essential oils among population individuals of this species in Iran D.S.S. (Determination of special station) method was used. In this study, the aerial parts of 5 individuals of Thymus eriocalyx were collected from 10 special stations, at full flowering stage for chemical investigation and were dried at room temperature. The essential oil was extracted by hydrodistilation method using a Clevenger-type apparatus. The chemical composition of essential oils was analyzed by GC-FID and GC-MS. Results showed that the highest average yield of essential oil in three replications based on dry weight belonged to the first individual of Te2 population (4.09%), while the lowest was recorded for the second individual of Te10 population (0.21%). Totally, thirty one compounds were identified in the essential oil of 10 studied population individuals. In this study, it is noteworthy that the individuals of a population showed variation among themselves in terms of chemical compositions. Among existing chemical compounds in the essential oil of 50 individuals, the following compounds revealed the variation widely: p-cymene (0.1-20.1%), 1,8-cineole (0.4-29.4%), γ-terpinene (0.1-8.7%), linalool (0.1-82.3%), camphor (0.1-15%), borneol (1.6-22.7%), geraniol (0.1-74.6%), thymol (0.1-57.7%), geranyl acetate (0.1-49.6%), caryophyllene oxide (0.2-9.1%), limonene (0.2-24%) and α-terpineol (0.1-36.2%). The results of the analysis of chemical data using MVSP Software by UPGMA and PCO methods led to the identification of 9 chemotypes as follows of which 7 chemotypes were introduced as new chemotypes: 1-geraniol/geranyl acetate, 2-geraniol, 3- linalool/geraniol/geranyl acetate, 4-linalool,5-α-terpineol/1,8-cineole, 6-geranyl acetate, 7-geraniol/thymol/borneol/1,8-cineole, 8-thymol/limonene and 9-thymol/p-cymene/borneol.
R. Kalvandi; M. Mirza; K. Safikhani; M. Naderi
Abstract
Rhabdosciadium aucheri Boiss., belonging to Apiaceae family, is an endemic species in flora of Iran that grows in Hamadan, Lorestan, Chaharmahale Bakhteyari, Kohkeyloye va Boyrahmad and Tehran. Aerial parts of Rhabdosciadium aucheri Boiss were collected at full flowering stage on July, 2010 from Iran: ...
Read More
Rhabdosciadium aucheri Boiss., belonging to Apiaceae family, is an endemic species in flora of Iran that grows in Hamadan, Lorestan, Chaharmahale Bakhteyari, Kohkeyloye va Boyrahmad and Tehran. Aerial parts of Rhabdosciadium aucheri Boiss were collected at full flowering stage on July, 2010 from Iran: Hamadan, Asadabad at an altitude of 2330 m. Then, 100 g of air-dried aerial parts of plant was subjected to hydrodistillation. The essential oil yield was 0.67% based on the dry weight of plant. The oil was analyzed by GC and GC/Mass. Totally, eighteen constituents were identified. β-sesquiphellandrene (49.9%), epi-α-bisabolol (11.9%), intermedeol (8.20%), germacrene D (7.6%) and β-bisabolene (4.9%) were identified as the major constituents. This research was carried out for the first time in the world.
Z. Dehghan; F. Sefidkon; Gh. Bakhshi Khaniki; R. Kalvandi
Abstract
Different species from Labiatea family are used as spices and fragrance in food or as medicine for treatment of viral or microbial diseases in many countries. Labiatea family contains 46 genus and 410 species. One of the valuable and medicinal species of this family is Ziziphora clinopodioides Lam. subsp. ...
Read More
Different species from Labiatea family are used as spices and fragrance in food or as medicine for treatment of viral or microbial diseases in many countries. Labiatea family contains 46 genus and 410 species. One of the valuable and medicinal species of this family is Ziziphora clinopodioides Lam. subsp. rigida (Boiss.). This species contains essential oil with pulegone as major compound. In this investigation, the effect of some ecological factors on essential oil content and composition of Z. clinopodioides was studied. The aerial parts of Z. clinopodioides have been collected at flowering stage from four habitats in Hamedan and Kurdestan provinces. The major parameters like altitude, side and slope percentage, soil characteristics and other species of plant were determined. The plant materials have been subjected to hydro-distillation in three replications to obtain their essential oils. The essential oils were analyzed by GC and GC/MS. The results showed that ecological factors had significant effect (a=0.01) on oil yields. Identification of chemical constituents of the oils showed that main compounds (pulegone and 1,8-cineole) and minor compounds exist in different percentage in different habitats. Therfore, the ecological factors affected essential oil composition of Z. clinopodioides.
F. Sefidkon; R. Kalvandi; M. Mirza
Volume 19, Issue 3 , October 2003, , Pages 255-267
Abstract
The genus of Nepeta presents 67 species in Iran, most of them are endemic. One of theses endemic species is N. heliotropifolia. In this research the essential oil of N. heliotropifolia was investigated in three different stage of plant growth. The aerial parts of N. heliotropifolia at before flowering, ...
Read More
The genus of Nepeta presents 67 species in Iran, most of them are endemic. One of theses endemic species is N. heliotropifolia. In this research the essential oil of N. heliotropifolia was investigated in three different stage of plant growth. The aerial parts of N. heliotropifolia at before flowering, beginning of flowering and complete flowering were collected from Hamedan province. Essential oils isolated by hydro-distillation from the aerial parts of three samples, were analyzed by a combination of capillary GC and GC/MS. The yields of essential oils were obtained 0.75%, 0.63% and 0.4% (w/w) respectively. So the oil yield of N. heliotropifolia was decreased from before flowering stage to complete flowering stage. Thirty-six components were identified in the oils. The main constituent of the essential oil at before flowering stage was limonene (40.1%), that decreased by plant growth. The second main component of the oil in this stage was b-pinene (11.1%), that increased to 23.3% in the beginning of flowering, then decreased in fool flowering. The other main compounds of the oils were 1,8-cineole, b-caryophyllene, (Z)- b-farnesene, g-mmurolene abd bicyclogermacrene.
R. Kalvandi; M. Atri; K. Safikhani
Volume 3, Issue 1 , April 1999, , Pages 107-131
